Journal: PLoS ONE

Article Title: Matriptase Deletion Initiates a Sjögren’s Syndrome-Like Disease in Mice

doi: 10.1371/journal.pone.0082852

Figure Lengend Snippet: Electrical potential (EP) of salivary glands was measured 22 weeks post-AAV2 delivery to glands in live AAV2-Cre ( N = 5) and AAV2-LacZ ( N = 3) mice. Membrane potential of pierced ducts of St14 LoxP/LoxP mice ( N = 2) was used as a control for impaired ductal epithelium. (A) Local depletion of matriptase in SMG from AAV2-Cre animals resulted in a significant EP decrease compared with SMG from AAV2-LacZ control mice. The EP of AAV2-Cre mice was similar to that of pierced glands. (B) Correlation between saliva production and EP of salivary glands from AAV2-Cre mice ( N = 3) and AAV2-LacZ mice ( N = 3). Data shown are the mean ± SEM for each group. Statistical significance was determined using unpaired Student’s t test and Pearson’s rank correlation test, respectively. NS, P = 0.1978, ** P = 0.0044, *** P <0.001. (C) Changes in distribution of proteins associated with tight junctions or salivary gland function induced by the loss of matriptase were visualized by immunofluorescent staining of paraffin-embedded SMG tissue samples from St14 – and St14 + control mice 28 to 40 weeks of age ( N = 2 both groups). Representative confocal images are shown. Apical staining of ductal cells for claudin 3 is shown by arrowheads in both St14 + and St14 − animals. An increase in the signal for claudin 3 was detected in the cytoplasm and basal membrane of ductal cells in St14 – mice compared with St14 + mice, as indicated with arrow; original magnification 40X.

Article Snippet: Correlation of SFR and EP was analyzed by Pearson’s rank correlation test (GraphPad Software Inc., La Jolla, CA, USA).

Techniques: Membrane, Control, Staining

Journal: Cancer Cell International

Article Title: A pan-cancer analysis of molecular characteristics and oncogenic role of gasdermins

doi: 10.1186/s12935-022-02483-4

Figure Lengend Snippet: The expression of GSDM family genes. A Box plots represent the relative expression of GSDM genes in pan-cancer. B Heatmap showing differential expression of GSDM genes across cancer types. C Matrix graph of Pearson’s correlation of GSDM genes expression in pan-cancer. D – I Box plots represented the differential expression of six GSDM genes between tumor and normal tissue. J Methylation difference between tumor and paired normal tissue. P value was adjusted by FDR, FDR < = 0.05 was considered as significant. Only significant results were shown as bubble plots. K Pearson’s Product-moment Correlation between GSDM genes expression and corresponding gene methylation. P-value was adjusted by FDR and genes with FDR < = 0.05 will be showed

Article Snippet: The correlated genes of GSDM family members were determined by the Pearson’s Product-moment Correlation test in colorectal cell lines based on Broad Institute Cancer Cell Line Encyclopedia (CCLE) dataset.

Techniques: Expressing, Quantitative Proteomics, Methylation

Journal: Cancer Cell International

Article Title: A pan-cancer analysis of molecular characteristics and oncogenic role of gasdermins

doi: 10.1186/s12935-022-02483-4

Figure Lengend Snippet: GSDM family was involved in promoting metastasis and angiogenesis of colorectal cancer. A These three-circle Venn diagrams showed the interaction between the correlated genes of GSDMA, DSDMB and GSDME. These four-circle Venn diagrams showed the interaction between the correlated genes of GSDMA, DSDMB, GSDME and GSDMD. Notice that there was no or almost no interaction between some groups. The correlated genes of GSDM family members were determined by the Pearson’s Product-moment Correlation test in colorectal cell lines based on Broad Institute Cancer Cell Line Encyclopedia (CCLE) dataset. GSDMA/B/D/E cor (+)/(−) denoted the genes positively/negatively significantly (P < 0.05) correlated with GSDMA/B/D/E. The GO enrichment analysis was performed on the genes correlated with all the four GSDM genes. B GSDME expression was positively correlated with the expression of CD31 and CD34 based on TCGA-COAD dataset analysis. C Increased expression of GSDME provided endothelial development advantage based on Gene Set Enrichment Analysis (GSEA). D Violin plot revealed that GSDME expression was correlated with stage of CRC

Article Snippet: The correlated genes of GSDM family members were determined by the Pearson’s Product-moment Correlation test in colorectal cell lines based on Broad Institute Cancer Cell Line Encyclopedia (CCLE) dataset.

Techniques: Expressing

Journal: Nature Communications

Article Title: Evidence for fungi and gold redox interaction under Earth surface conditions

doi: 10.1038/s41467-019-10006-5

Figure Lengend Snippet: Pearson correlation between inverse Simpson index and in situ gold concentrations

Article Snippet: Differences and correlation between two sets of data were determined to be significant using an unpaired two-tailed t -test and Pearson correlation coefficient (GraphPad Prism Version 7; GraphPad Software, La Jolla, CA, USA).

Techniques: In Situ, Bacteria